Beetroot has a bit of an identity crisis. For most of the 20th century, it was just pickled slices sitting on a plate next to a burger, faintly vinegary and largely ignored. Then, around 2007, a group of researchers at the University of Exeter published a study showing that dietary nitrate โ found in high concentrations in beetroot โ could meaningfully reduce the oxygen cost of exercise. The supplement industry took note. The sports nutrition world took note. And suddenly, beet juice shots started appearing in the bags of marathon runners and Tour de France pelotons.
Since then, the research has genuinely exploded. A 2025 systematic review in Nutrients that surveyed studies from 2020 to 2025 confirmed that beetroot-derived nitrates improve endurance, oxygen efficiency, muscular power, and cardiovascular function in regular people โ though results in elite athletes are more mixed, likely because their bodies already process nitric oxide more efficiently. A separate 2025 ScienceDirect review covering a full decade of research found clinical trial support for improvements in blood pressure, blood sugar control, and exercise performance across multiple populations including people with diabetes, hypertension, and even COVID-19 recovery patients.
So what do we actually know? Let's go through the evidence properly โ not hype, not scaremongering, just what the studies show.
What's Actually Inside a Beetroot?
Before diving into benefits, it's worth knowing what you're working with. A 100g serving of raw beetroot contains approximately 43 calories โ virtually nothing โ alongside a surprisingly comprehensive nutritional profile. It's classed as "high in folate" under EU nutritional regulations, meaning a single serving gets you halfway to your daily requirement. The NHS lists beetroot under its 5-a-day guidance (7 slices counts as one portion), and the vegetable's folate content is something they specifically highlight for pregnant women, as B9 plays a critical role in foetal neural tube development.
| Nutrient | Per 100g raw | % Daily Value |
|---|---|---|
| Calories | 43 kcal | โ |
| Carbohydrates | 9.1g | โ |
| Dietary Fibre | 2.5g | ~10% |
| Protein | 1.6g | โ |
| Fat | 0.1g | โ (fat-free) |
| Folate (B9) | 100โ109ฮผg | ~50% DV |
| Potassium | 325mg | ~15% DV |
| Manganese | 0.33mg | ~15% DV |
| Vitamin C | 10mg | ~10% DV |
| Iron | 0.9mg | ~5% DV |
| Magnesium | 25mg | ~6% DV |
| Inorganic Nitrate | ~250mg | โ |
Beyond the standard vitamins and minerals, beetroot contains a class of bioactive compounds found in very few other foods โ the betalains. These are worth understanding properly because they're where most of the more exciting research is focused.
The Molecule That Makes It Red โ and Why It Matters
Betanin โ known in food labelling as E162 and listed in PubChem's compound database under CID 6540685 with molecular formula CโโHโโNโOโโ โ is the primary pigment responsible for beetroot's deep crimson colour. It's the most studied betalain pigment on earth, and what makes it unusual compared to most plant pigments is that it contains nitrogen in its structure. That's rare. Most red plant pigments (like anthocyanins in berries) don't have nitrogen at all. Betanin is derived from the amino acid tyrosine, which is also the precursor to dopamine and adrenaline โ though that connection is somewhat tangential to its health effects.
Molecular formula: CโโHโโNโOโโ ยท Molecular weight: 550.47 g/mol
Classification: Betacyanin ยท Glycoside (betanidin 5-O-ฮฒ-D-glucopyranoside)
Beetroot concentration: 300โ600 mg/kg
Also found in: Swiss chard, Opuntia cactus, amaranth leaves
Approved: FDA (21 CFR Section 73.40) + EU (E162) as natural food colourant ยท No safety concerns identified
What makes betanin genuinely interesting from a health perspective is its free radical scavenging activity. Research published in PMC found that its antioxidant ability is nearly twice that of some anthocyanins at pH above 4 โ meaning in the mildly acidic environment of most foods and your upper digestive tract, it outperforms the red pigments found in blueberries, cherries, and red wine. The mechanism, according to a 2022 study in PMC, involves something called the SPLET pathway โ Sequential Proton Loss Electron Transfer โ which is how it neutralises reactive oxygen and nitrogen species (free radicals) at a molecular level.
One important caveat that's often missed: betanin is heat-sensitive and light-sensitive. Prolonged boiling destroys it. If you want its antioxidant benefits, raw beetroot, lightly roasted, or steamed is significantly better than boiling. This is why nutritionists at Northwestern Medicine specifically recommend steaming, roasting, and grilling over boiling. Betanin also survives pasteurisation reasonably well when in high-sugar environments, which is why commercial beetroot juice tends to retain a fairly good red colour.
The 7 Evidence-Based Benefits
๐ It Genuinely Lowers Blood Pressure
This is the most robustly supported benefit in the research. The mechanism is well understood: dietary nitrate in beetroot is converted by bacteria in your mouth into nitrite, which your body then converts to nitric oxide โ a signalling molecule that causes blood vessels to relax and widen. The result is reduced blood pressure, particularly in older adults and people with hypertension. NHS Research Authority-registered trials at Royal Brompton Hospital, King's College Hospital, and St Mary's Manchester have all used beetroot juice as an active intervention, specifically because the evidence is strong enough to justify clinical testing. The Royal Brompton COPD trial found a sustained 4.5mmHg reduction in systolic blood pressure after 90 days of 70ml daily beetroot shots. A 2025 systematic review covering the previous decade found blood pressure improvements across healthy individuals, hypertensive patients, and even those in COVID-19 recovery. It won't replace medication, and the NHS is clear that you should never stop prescribed blood pressure drugs without speaking to a doctor โ but as a dietary addition, the evidence is legitimate.
๐ It Improves Exercise Performance (In Regular People)
The 2007 Exeter study that started this whole area of research has been replicated many times over. The effect is real but has an important asterisk: it works much better for people with low to moderate fitness than for elite athletes. An umbrella review published in June 2025 โ covering meta-analyses from seven major databases including PubChem, Cochrane, and CINAHL from 2000 to 2024 โ found that beetroot juice consistently improved endurance, oxygen efficiency, and muscular force in recreational exercisers, but produced little to no effect in highly trained athletes. The explanation is that elite athletes have already optimised their nitric oxide pathways through training. For everyone else โ people who jog, cycle, do gym classes โ the evidence suggests a meaningful improvement in how far and how hard you can push before hitting fatigue. A 2024 study specifically found that 70ml of juice improved aerobic exercise capacity and cardiovascular function in healthy men. The NHS-registered COPD trial also measured walking distance as an outcome, finding a 30-metre improvement in six-minute walk tests โ a clinically meaningful gain for people with a chronic lung condition.
๐ง It May Support Brain Health as You Age
As people get older, the body produces less nitric oxide naturally. Less nitric oxide means reduced blood flow โ including to the brain. This is now considered one of the contributing factors to age-related cognitive decline and reduced processing speed. The December 2025 systematic review in Nutrients specifically examined the cognitive dimension of beetroot supplementation and found that while the evidence is still early and "limited and inconsistent," there are plausible biological mechanisms and some positive human studies to support the hypothesis. Separately, a 2024 randomised crossover clinical trial published in European Journal of Nutrition tested a chewable beetroot-based supplement and found acute improvements in cognitive performance. Northwestern Medicine's dietitians summarise it well: when combined with exercise, nitrates from beetroot juice can increase blood flow to the brain during cognitive tasks. This remains an emerging area, but the direction of evidence is encouraging.
๐ก๏ธ Its Antioxidants Fight Inflammation at a Cellular Level
The betalain family โ primarily betanin (E162) and the yellow vulgaxanthin โ are classified as strong antioxidants in their own right. Research published in PMC found that betanin can protect DNA against hydrogen peroxide-induced damage in human cells, reduce reactive oxygen species (ROS), activate the Nrf2 antioxidant response pathway (a key regulator of cellular defence), and increase glutathione levels. Glutathione is arguably the body's most important endogenous antioxidant โ the fact that a food compound can upregulate it is notable. The broader context is that chronic low-grade inflammation is the underlying driver of most major diseases โ cardiovascular disease, type 2 diabetes, some cancers, and neurodegeneration. Beetroot doesn't cure any of these. But as part of a consistently vegetable-rich diet, its anti-inflammatory compounds contribute to the systemic protective effect that epidemiologists consistently find when studying plant-heavy eating patterns.
๐ซ It's Good for Your Heart Beyond Just Blood Pressure
Beetroot's cardiovascular benefits extend beyond the nitrate-nitric oxide pathway. The folate content (B9) plays a direct role in controlling homocysteine levels โ elevated homocysteine is an independent risk factor for heart disease and stroke, and folate helps metabolise it away. The potassium content supports heart rhythm and counteracts the blood-pressure-raising effect of sodium. The betanin pigments also help reduce the oxidation of LDL cholesterol โ sometimes called "bad" cholesterol โ which, when oxidised, is one of the primary triggers of arterial plaque buildup. Additionally, a 2024 pilot RCT published in Clinical Nutrition found that beetroot juice positively influenced gut microbiota and inflammation markers in adults recovering from long COVID, suggesting systemic anti-inflammatory effects that benefit cardiovascular health broadly.
๐ฉบ It Has Liver-Protective Properties
Betanin has what researchers call "antihepatotoxic" properties โ essentially, it helps protect the liver. Multiple animal studies have found that it attenuates liver damage caused by toxic compounds including carbon tetrachloride. The mechanism involves stimulating quinone reductase II โ an enzyme the liver uses to neutralise harmful substances โ and reducing oxidative stress in hepatic cells. In human cell line studies, betanin has been shown to induce the translocation of the Nrf2 transcription factor into cell nuclei, which activates a suite of detoxifying enzymes including glutathione S-transferases and heme oxygenase-1. These are the liver's frontline defences. The research is still largely preclinical (meaning it's been done in cells and rats rather than in large human trials), but the biochemical case for a liver-supportive role is well-constructed. For context, the NHS specifically acknowledges beetroot as a liver-friendly food due to its betaine content, which helps protect liver cells from inflammation.
๐ฌ Early Research Suggests Possible Anticancer Properties
This is probably the benefit you should treat with the most scepticism โ not because the evidence doesn't exist, but because it's still mostly at the cell culture and animal study stage. What the research shows is that purified betanin inhibits the proliferation of melanoma cancer cells, and shows growth inhibition against breast (MCF-7), colon (HCT-116), stomach (AGS), CNS (SF-268), and lung (NCI-H460) cancer cell lines in lab conditions. It also induces apoptosis (programmed cell death) in human chronic myelogenous leukaemia cell lines. A 2019 review found evidence that betalain can disrupt the cancerous mutations of cells. A 2021 review in Natural Product Reports describes the antiproliferative mechanisms in considerable detail. What this absolutely does not mean is that eating beetroot prevents cancer. These are in-vitro findings, not clinical outcomes. But they're consistent enough that researchers are actively investigating whether betanin-based extracts could have roles as chemotherapy adjuncts, particularly given that it appears to enhance the efficacy of doxorubicin (a standard chemotherapy drug) against breast cancer cells.
What Nobody Tells You: The Side Effects
Let's talk about the thing that happens the first time you eat a lot of beetroot and don't know about it: your urine turns pink or red. It can also turn your stools red or dark. This is called beeturia, and it affects around 10โ14% of people. It's completely harmless โ it's just betanin passing through your system without being fully broken down โ but it has alarmed many people into thinking they have blood in their urine. If you've eaten a lot of beetroot in the last 24 hours, that's almost certainly what's going on.
Kidney stones: Beetroot is high in oxalates. If you're prone to calcium-oxalate kidney stones, talk to your doctor before making beetroot a daily staple.
Blood pressure medication: Because beetroot genuinely lowers blood pressure, people already taking antihypertensives should be careful about combining large amounts with their medication. Again, speak to your GP first.
Gout: Purines in beetroot can be a concern for people with gout, though the levels are relatively low compared to meat sources.
Beeturia: Pink or red urine after eating beetroot is normal and harmless. If you haven't eaten beetroot, bloody urine warrants a GP visit.
How to Actually Eat It for Benefits
The most studied form in clinical trials is concentrated beetroot juice โ typically a 70ml shot containing around 400mg of nitrate. That's what the NHS-registered trials used. But you don't need supplements. According to research cited by LoveBeetroot.co.uk, which references McCance and Widdowson nutritional composition data, 200g of cooked beetroot delivers equivalent health benefits to 500ml of beetroot juice. The NHS counts 7 slices or 3 small whole beetroots as one of your five-a-day portions.
Cooking method matters. Roasting and steaming preserve the betanin content significantly better than boiling. Prolonged boiling in water will leach both betanin and water-soluble nutrients including folate and vitamin C. If you do boil, use minimal water and short cooking times. Raw beetroot retains the highest nutrient profile overall, though it's harder to eat in large quantities. Grated raw into salads or blended into smoothies is practical.
One counterintuitive finding from the University of Exeter research: using antibacterial mouthwash before drinking beetroot juice significantly reduces its blood pressure benefits. The reason is that the mouth bacteria responsible for converting dietary nitrate to nitrite are killed by antibacterial rinses. Don't rinse before your beet shot if you're drinking it for cardiovascular reasons.
The Honest Bottom Line
Beetroot is not a superfood in the hype-laden sense that word usually implies. It's a vegetable โ one that happens to be unusually rich in a specific class of compounds (dietary nitrates and betalains) that have been studied more rigorously than almost any other vegetable over the past 20 years. The blood pressure evidence is strong. The exercise performance evidence is solid for non-elite exercisers. The antioxidant and anti-inflammatory mechanisms are well characterised. The liver protection and brain health evidence is promising but needs more human trial data.
The most important thing is probably the simplest one: the NHS's 5-a-day guidance is there for a reason, and very few people hit it. If eating beetroot regularly helps you get another portion of vegetables into your diet, you're already doing something good โ the specific bioactive compounds are a bonus. Add it to salads, blend it into soups, roast it with goat's cheese, or drink a small shot before a workout. It's inexpensive, widely available, and backed by more peer-reviewed research than almost any other root vegetable on the shelf.
Sources & References
- Nutrients (Dec 2025) โ Beetroot Juice Supplementation: Physical Performance and Cognitive Functions Systematic Review
- ScienceDirect (Dec 2025) โ A Decade of Beetroot Research 2015โ2025
- PMC โ Beetroot as a functional food: Antioxidant, antitumor, physical function and chronic metabolomics activity
- PMC โ The Potential Benefits of Red Beetroot Supplementation in Health and Disease
- PMC โ Biological Properties and Applications of Betalains
- PMC โ Betanin: Stability, Bioavailability, Antioxidant and Preservative Ability
- PMC โ Mechanism of Antioxidant Activity of Betanin and Betanidin
- PMC โ Effects of Beetroot Juice on Physical Performance: An Umbrella Review (2025)
- NHS Health Research Authority โ ON-BC Study: Beetroot Juice & Blood Pressure in COPD
- NHS Health Research Authority โ BEET-BP Trial: Dietary Nitrate in Pregnancy
- NHS 5-a-Day โ What counts as a portion of fruit and vegetables
- Royal Brompton Hospital NHS โ Beetroot juice lowers blood pressure for people with COPD
- PubChem CID 6540685 โ Betanin (CโโHโโNโOโโ)
- Natural Product Reports (2021) โ Multi-colored shades of betalains: advances in betacyanin chemistry
- MDPI Agriculture (Jan 2025) โ Red Beetroot: Phytochemicals, Extraction Methods, Health Benefits
- Clinical Nutrition (2024) โ Beetroot juice, gut microbiota and inflammation in long COVID
- McCance & Widdowson Composition of Foods Integrated Dataset, 2019